HDAC4 Flag
(Plasmid #13821)

• Purpose: Mammalian expression of human histone deacetylase 4 with flag tag
• Depositing Lab: Eric Verdin
• Publication: Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-20.

Backbone

• Vector backbone: pcDNA3.1 +
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HDAC4
• Alt name: Histone Deacetylase A
• Alt name: HDACA
• Species: H. sapiens (human)
• Insert Size (bp): 3252
• GenBank ID: NM_006037.2 NP_006028.1
• Entrez Gene: HDAC4 (a.k.a. AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, NEDCHF, NEDCHID)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 33 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).

The HDAC4 insert in this plasmid matches GenBank reference sequence NP_006028.1.