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Addgene

HDAC4 Flag
(Plasmid #13821)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13821 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1 +
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HDAC4
  • Alt name
    Histone Deacetylase A
  • Alt name
    HDACA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3252
  • GenBank ID
    NM_006037.2 NP_006028.1
  • Entrez Gene
    HDAC4 (a.k.a. AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, NEDCHF, NEDCHID)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).

The HDAC4 insert in this plasmid matches GenBank reference sequence NP_006028.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HDAC4 Flag was a gift from Eric Verdin (Addgene plasmid # 13821 ; http://n2t.net/addgene:13821 ; RRID:Addgene_13821)
  • For your References section:

    A new family of human histone deacetylases related to Saccharomyces cerevisiae HDA1p. Fischle W, Emiliani S, Hendzel MJ, Nagase T, Nomura N, Voelter W, Verdin E. J Biol Chem. 1999 Apr 23. 274(17):11713-20. 10.1074/jbc.274.17.11713 PubMed 10206986