pcDNA3.1-(empty)-TAG
(Plasmid #138209)

• Purpose: (Empty Backbone) To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region
• Depositing Lab: Neal Devaraj
• Publication: Busby et al Methods Enzymol. 2020;641:373-399. doi: 10.1016/bs.mie.2020.03.009. Epub 2020 Apr 20.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 5500
• Vector type: Mammalian Expression ; T7 Transcription
• Promoter: CMV Promoter, T7 Promoter
• Tag / Fusion Protein:
  • TAG sequence

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV Promoter

Resource Information

• Supplemental Documents:
  • pcDNA3.1-empty-TAG.gbk
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

An RNA of interest can be cloned into this vector in order to append a 25-nucleotide TGT recognition element to its 3' end. The TGT recognition element, or TAG, has short spacer arms at either side.