TGT-His
(Plasmid #138201)

• Purpose: Expresses E. Coli TGT with a C-terminal His Tag
• Depositing Lab: Neal Devaraj
• Publication: Busby et al Methods Enzymol. 2020;641:373-399. doi: 10.1016/bs.mie.2020.03.009. Epub 2020 Apr 20.

Backbone

• Vector backbone: pUCIDT-Kan
• Backbone manufacturer: IDT
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 4000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: E Coli tRNA Guanine Transglycosylase-His
• Alt name: TGT
• Species: E. Coli
• Insert Size (bp): 1146
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: T7 Promoter

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Seth Alexander

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

J. Am. Chem. Soc. 2015, 137, 40, 12756-12759