HDAC1 Flag
(Plasmid #13820)

• Purpose: Mammalian expression of human histone deacetylase 1 with flag tag
• Depositing Lab: Eric Verdin
• Publication: Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 95(6):2795-800.

Backbone

• Vector backbone: pcDNA3.1 +
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HDAC1
• Alt name: Histone Deacetylase 1
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• GenBank ID: NM_004964
• Entrez Gene: HDAC1 (a.k.a. GON-10, HD1, KDAC1, RPD3, RPD3L1)
• Tag / Fusion Protein:
  • flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH Reverse

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
  • Addgene Diagnostic Digest
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 39 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).