CDKN1A sgRNA
(Plasmid #138189)

• Purpose: 3rd generation lentiviral gRNA plasmid targeting human CDKN1A
• Depositing Lab: Wen Xue
• Publication: Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/hep.30807.

Backbone

• Vector backbone: LentiCRISPRv2 hygro
• Backbone manufacturer: Brett Stringer (Addgene plasmid #98291)
• Total vector size (bp): 15261
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CDKN1A
• Alt name: p21
• gRNA/shRNA sequence: CACCGTCACCGAGACACCACTGGA
• Species: H. sapiens (human)
• GenBank ID: NG_009364.1 NM_000389.5
• Entrez Gene: CDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (destroyed during cloning)
• 3′ cloning site: BsmB1 (destroyed during cloning)
• 5′ sequencing primer: hU6-F (5'-GAGGGCCTATTTCCCATGATT-3')

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry