pMGS59(AID-ARF-P2A-Hygromycin)
(Plasmid
#138174)
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PurposeAn AID-ARF-P2A-Hygromycin fusion cassette to tag the protein of interests with auxin-inducible degron and ARF at the c-terminal
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57
- Total vector size (bp) 4873
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAID-ARF-P2A-Hygromycin
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SpeciesOryza sativa
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Tag
/ Fusion Protein
- AID-ARF clamp degron tag (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWe synthesized the insert from Bio Basic.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Pre-print- arXiv:2002.12883
https://arxiv.org/abs/2002.12883
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMGS59(AID-ARF-P2A-Hygromycin) was a gift from Michael Guertin (Addgene plasmid # 138174 ; http://n2t.net/addgene:138174 ; RRID:Addgene_138174) -
For your References section:
ARF-AID: A Rapidly Inducible Protein Degradation System That Preserves Basal Endogenous Protein Levels. Sathyan KM, Scott TG, Guertin MJ. Curr Protoc Mol Biol. 2020 Sep;132(1):e124. doi: 10.1002/cpmb.124. 10.1002/cpmb.124 PubMed 32757370
Map uploaded by the depositor.
