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Addgene

EE-BE4max
(Plasmid #138158)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322/CMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rAPOBEC1(EE)-nSpCas9-UGI-UGI
  • Species
    Synthetic; fusion of genes derived from R. norvegicus, S. pyogenes, and Bacillus phage PBS2
  • Mutation
    Within rAPOBEC1 R126E + R132E; within Cas9 D10A
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EE-BE4max was a gift from David Liu (Addgene plasmid # 138158 ; http://n2t.net/addgene:138158 ; RRID:Addgene_138158)

  • For your References section:

    Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Doman JL, Raguram A, Newby GA, Liu DR. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. 10.1038/s41587-020-0414-6 PubMed 32042165
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