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pT7NT*-Bri2 113-231 R221E
(Plasmid #138134)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138134 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pET vector
  • Backbone size w/o insert (bp) 5647
  • Total vector size (bp) 6004
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Bri2 BRICHOS R221E
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    357
  • Entrez Gene
    ITM2B (a.k.a. ABRI, BRI, BRI2, BRICD2B, E25B, E3-16, FBD, RDGCA, imBRI2)
  • Promoter T7
  • Tag / Fusion Protein
    • NT* tag derived from spider silk proteins (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer T7 terminator primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7NT*-Bri2 113-231 R221E was a gift from Jan Johansson (Addgene plasmid # 138134 ; http://n2t.net/addgene:138134 ; RRID:Addgene_138134)

  • For your References section:

    Augmentation of Bri2 molecular chaperone activity against amyloid-beta reduces neurotoxicity in mouse hippocampus in vitro. Chen G, Andrade-Talavera Y, Tambaro S, Leppert A, Nilsson HE, Zhong X, Landreh M, Nilsson P, Hebert H, Biverstal H, Fisahn A, Abelein A, Johansson J. Commun Biol. 2020 Jan 20;3(1):32. doi: 10.1038/s42003-020-0757-z. 10.1038/s42003-020-0757-z PubMed 31959875
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