pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody
(Plasmid #138132)

• Purpose: Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.
• Depositing Lab: Kazuhisa Nakayama
• Publication: Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250. doi: 10.1091/mbc.E20-04-0250.

Backbone

• Vector backbone: pGEX6P1
• Backbone manufacturer: GE healthcare
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6091
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EGFP-anti-mouse-IgG(kappa)-nanobody
• Alt name: TP1172
• Species: Vicugna pacos
• Insert Size (bp): 1125
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CGTATTGAAGCTATCCCAC
• 3′ sequencing primer: CGGCATCCGCTTACAGAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid #104161.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry