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Addgene

pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody
(Plasmid #138129)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138129 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX6P1
  • Backbone manufacturer
    GE healthcare
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6061
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mClover3-anti-rabbit-IgG(Fc)-nanobody
  • Alt name
    TP955
  • Species
    Vicugna pacos
  • Insert Size (bp)
    1095
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CGTATTGAAGCTATCCCAC
  • 3′ sequencing primer CGGCATCCGCTTACAGAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #74253 and #104164.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody was a gift from Kazuhisa Nakayama (Addgene plasmid # 138129 ; http://n2t.net/addgene:138129 ; RRID:Addgene_138129)

  • For your References section:

    Practical method for super-resolution imaging of primary cilia and centrioles by expansion microscopy using an amplibody for fluorescence signal amplification. Katoh Y, Chiba S, Nakayama K. Mol Biol Cell. 2020 Jul 29:mbcE20040250. doi: 10.1091/mbc.E20-04-0250. 10.1091/mbc.E20-04-0250 PubMed 32726175
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