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Addgene

pYPQ289-RVR
(Plasmid #138127)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138127 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ167
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible Cas12a entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ErCas12a RVR variant
  • Species
    Eubacterium rectale
  • Mutation
    D513R, K519V and N523R
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ289-RVR was a gift from Yiping Qi (Addgene plasmid # 138127 ; http://n2t.net/addgene:138127 ; RRID:Addgene_138127)

  • For your References section:

    Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems. Zhang Y, Ren Q, Tang X, Liu S, Malzahn AA, Zhou J, Wang J, Yin D, Pan C, Yuan M, Huang L, Yang H, Zhao Y, Fang Q, Zheng X, Tian L, Cheng Y, Le Y, McCoy B, Franklin L, Selengut JD, Mount SM, Que Q, Zhang Y, Qi Y. Nat Commun. 2021 Mar 29;12(1):1944. doi: 10.1038/s41467-021-22330-w. 10.1038/s41467-021-22330-w PubMed 33782402
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