pPS2189
(Plasmid
#1381)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1381 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPS1304
- Backbone size w/o insert (bp) 5200
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGal-Clb2-GFP
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Alt nameClb2
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1400
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Entrez GeneCLB2 (a.k.a. YPR119W)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pPS1304 has the GAL1 promoter (EcoRI/XbaI fragment) and GFP (HindIII fragment) in YEp352 (2 micron ori).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS2189 was a gift from Pamela Silver (Addgene plasmid # 1381 ; http://n2t.net/addgene:1381 ; RRID:Addgene_1381) -
For your References section:
The Saccharomyces cerevisiae cyclin Clb2p is targeted to multiple. Hood JK, Hwang WW, Silver PA. J Cell Sci 2001 Feb;114(Pt 3):589-97. PubMed 11171327