pLenti-Tet-GBP1-C589A
(Plasmid #138090)

• Purpose: Lentiviral backbone for inducible expression of human GBP1 missing the reactive cysteine of the CaaX-box (GBP1-C589A)
• Depositing Lab: Eva Frickel
• Publication: Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi: 10.15252/embj.2018100926. Epub 2019 Jun 3.

Backbone

• Vector backbone: Custom
• Backbone size w/o insert (bp): 8681
• Total vector size (bp): 10460
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human GBP1
• Species: H. sapiens (human)
• Insert Size (bp): 1779
• Mutation: cysteine 589 to alanine (C589A)
• Entrez Gene: GBP1
• Promoter: minimal CMV promoter with heptamerized tet‐operator

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TCCACGCTGTTTTGACCTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid can be used in conjunction with pTet-CTRL (Addgene #138085) that expresses the Tet-transactivator proteins for controlled expression of GBP1 missing the reactive cysteine of the CaaX-box (C589A) from this plasmid. Following Lentiviral transduction into mammalian cells expression can be induced with Tetracycline or Doxycyline.