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Addgene

pGene-TAP-GBP1
(Plasmid #138087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGene
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4624
  • Total vector size (bp) 6577
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human GBP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1953
  • Entrez Gene
    GBP1
  • Promoter Gal4-E1b
  • Tag / Fusion Protein
    • Tandem affinity purification (TAP) tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (unknown if destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CTGCTATTCTGCTCAACCT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGene-TAP-GBP1 was a gift from Eva Frickel (Addgene plasmid # 138087 ; http://n2t.net/addgene:138087 ; RRID:Addgene_138087)

  • For your References section:

    Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis. Fisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, Frickel EM. EMBO J. 2019 Jul 1;38(13):e100926. doi: 10.15252/embj.2018100926. Epub 2019 Jun 3. 10.15252/embj.2018100926 PubMed 31268602
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