pLenti-Tet
(Plasmid #138086)

• Purpose: (Empty Backbone) Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles
• Depositing Lab: Eva Frickel
• Publication: Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi: 10.15252/embj.2018100926. Epub 2019 Jun 3.

Backbone

• Vector backbone: Custom
• Backbone size (bp): 8684
• Vector type: Mammalian Expression, Lentiviral
• Promoter: minimal CMV promoter with heptamerized tet‐operator
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: TCCACGCTGTTTTGACCTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid can be used in conjunction with pTet-CTRL (Addgene #138085) that expresses the Tet-transactivator proteins for controlled expression of the ORF for the gene of interest cloned into this plasmid. Following Lentiviral transduction into mammalian cells expression can be induced with Tetracycline or Doxycyline.
To clone the gene of interest into this plasmid, digest with BamHI and then perform Gibson assembly, adding your gene of interest including any tags.