Skip to main content
Addgene

pTet-CTRL
(Plasmid #138085)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138085 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Custom
  • Backbone size w/o insert (bp) 7821
  • Total vector size (bp) 9807
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TetON-P2A-TetOFF-T2A-bsd
  • Insert Size (bp)
    1986
  • Promoter EF1a core promoter

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Both parts of the insert originate from plasmids from Addgene: "rtTA2S‐M2" TetON transactivator was cloned from Addgene #62346 (Hiroshi Ochiai) and "tTS" TetOFFtransactivator was cloned from Addgene #12390 (Zuoshang Xu).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid can be used in conjunction with pLenti-Tet (Addgene #138086) that has the ORF for the gene of interest of which expression can then be induced with Tetracycline or Doxycyline in Mammalian cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTet-CTRL was a gift from Eva Frickel (Addgene plasmid # 138085)

  • For your References section:

    Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis. Fisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, Frickel EM. EMBO J. 2019 Jul 1;38(13):e100926. doi: 10.15252/embj.2018100926. Epub 2019 Jun 3. 10.15252/embj.2018100926 PubMed 31268602
Related items: