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Addgene

pWZL Blast ST
(Plasmid #13805)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13805 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pWZL-Blast
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SV40 small T antigen
  • Alt name
    small T cDNA
  • Species
    simian virus
  • Insert Size (bp)
    550
  • Entrez Gene
    SV40gp7 (a.k.a. SV40gp7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZL Blast ST was a gift from William Hahn (Addgene plasmid # 13805 ; http://n2t.net/addgene:13805 ; RRID:Addgene_13805)
  • For your References section:

    Identification of specific PP2A complexes involved in human cell transformation. Chen W, Possemato R, Campbell KT, Plattner CA, Pallas DC, Hahn WC. Cancer Cell. 2004 Feb . 5(2):127-36. 10.1016/S1535-6108(04)00026-1 PubMed 14998489