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Addgene

MYMH 29
(Plasmid #138029)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138029 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA-331Bb
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pSEVA-331Bb-5AC-GFP
  • Species
    Synthetic
  • Mutation
    AAA GCG GAG AAA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Low expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MYMH 29 was a gift from Meng How Tan (Addgene plasmid # 138029 ; http://n2t.net/addgene:138029 ; RRID:Addgene_138029)

  • For your References section:

    An Expanded Synthetic Biology Toolkit for Gene Expression Control in Acetobacteraceae. Teh MY, Ooi KH, Danny Teo SX, Bin Mansoor ME, Shaun Lim WZ, Tan MH. ACS Synth Biol. 2019 Apr 19;8(4):708-723. doi: 10.1021/acssynbio.8b00168. Epub 2019 Mar 22. 10.1021/acssynbio.8b00168 PubMed 30865830
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