MYMH 13
(Plasmid
#138021)
-
PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 138021 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepSEVA-331Bb
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namepSEVA-331Bb-2AC-GFP
-
SpeciesSynthetic
-
MutationACA GAG GAG AAA
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
High expression
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MYMH 13 was a gift from Meng How Tan (Addgene plasmid # 138021 ; http://n2t.net/addgene:138021 ; RRID:Addgene_138021) -
For your References section:
An Expanded Synthetic Biology Toolkit for Gene Expression Control in Acetobacteraceae. Teh MY, Ooi KH, Danny Teo SX, Bin Mansoor ME, Shaun Lim WZ, Tan MH. ACS Synth Biol. 2019 Apr 19;8(4):708-723. doi: 10.1021/acssynbio.8b00168. Epub 2019 Mar 22. 10.1021/acssynbio.8b00168 PubMed 30865830