pTJK475
(Plasmid
#138014)
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PurposeSIX1 CRISPRi
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 138014 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTJK459
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable ; Stellar Cells
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSIX1 sgRNA13
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gRNA/shRNA sequenceTGGATGCTGCTAGTTGCCG
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SpeciesH. sapiens (human)
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MutationNone
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Entrez GeneSIX1 (a.k.a. BOS3, DFNA23, TIP39)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmB1 (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTJK475 was a gift from Tami Kingsbury (Addgene plasmid # 138014 ; http://n2t.net/addgene:138014 ; RRID:Addgene_138014)