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Addgene

pTJK635
(Plasmid #138009)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138009 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX459
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable ; Stellar Cells
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SIX1 sgRNA2
  • gRNA/shRNA sequence
    GCTGAAGGCGCATTACGTGG
  • Species
    H. sapiens (human)
  • Mutation
    None
  • Entrez Gene
    SIX1 (a.k.a. BOS3, DFNA23, TIP39)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bbs1 (unknown if destroyed)
  • 3′ cloning site Bbs1 (unknown if destroyed)
  • 5′ sequencing primer GAGGGCCTATTTCCCATGATT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTJK635 was a gift from Tami Kingsbury (Addgene plasmid # 138009 ; http://n2t.net/addgene:138009 ; RRID:Addgene_138009)
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