pTJK459
(Plasmid
#138008)
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Purpose(Empty Backbone) Empty sgRNA expression vector for expression of sgRNA for Sp Cas9
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 138008 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAddgene 62207
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable ; Stellar Cells
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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MutationU-A flip and hairpin extension: cgtttAagagctaTGCTGgaaaCAGCAtag-caagttTaaata
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTJK459 was a gift from Tami Kingsbury (Addgene plasmid # 138008 ; http://n2t.net/addgene:138008 ; RRID:Addgene_138008)