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Addgene

pTJK438
(Plasmid #138004)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138004 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWCC43
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable ; Stellar Cells
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    3xMYC tagged SIX1
  • Species
    H. sapiens (human)
  • Mutation
    None
  • Entrez Gene
    SIX1 (a.k.a. BOS3, DFNA23, TIP39)
  • Tag / Fusion Protein
    • 3xMYC (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (unknown if destroyed)
  • 3′ cloning site Sal1 (unknown if destroyed)
  • 5′ sequencing primer TTCATTCTCAAGCCTCAGAC
  • 3′ sequencing primer GGACGCTCGCTGCGCCCTTCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTJK438 was a gift from Tami Kingsbury (Addgene plasmid # 138004 ; http://n2t.net/addgene:138004 ; RRID:Addgene_138004)
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