pMIG AS B56 gamma3
(Plasmid
#13800)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMIG
- Backbone size w/o insert (bp) 6500
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Vector typeMammalian Expression, Retroviral
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnti-sense PP2A B56 gamma3
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Alt namePPP2R5C
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SpeciesH. sapiens (human)
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Insert Size (bp)1350
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MutationAnti-sense
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Entrez GenePPP2R5C (a.k.a. B56G, B56gamma, PR61G)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMIG AS B56 gamma3 was a gift from William Hahn (Addgene plasmid # 13800 ; http://n2t.net/addgene:13800 ; RRID:Addgene_13800) -
For your References section:
Identification of specific PP2A complexes involved in human cell transformation. Chen W, Possemato R, Campbell KT, Plattner CA, Pallas DC, Hahn WC. Cancer Cell. 2004 Feb . 5(2):127-36. 10.1016/S1535-6108(04)00026-1 PubMed 14998489
Map uploaded by the depositor.