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pET15b-mLin28A-cHIS
(Plasmid #137998)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137998 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse Lin28A, CSD and ZnFs (residues 36–186)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    500
  • Tag / Fusion Protein
    • C-terminal His6 tag

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-mLin28A-cHIS was a gift from Judy Lieberman (Addgene plasmid # 137998 ; http://n2t.net/addgene:137998 ; RRID:Addgene_137998)

  • For your References section:

    An RNA-binding Protein, Lin28, Recognizes and Remodels G-quartets in the MicroRNAs (miRNAs) and mRNAs It Regulates. O'Day E, Le MT, Imai S, Tan SM, Kirchner R, Arthanari H, Hofmann O, Wagner G, Lieberman J. J Biol Chem. 2015 Jul 17;290(29):17909-22. doi: 10.1074/jbc.M115.665521. Epub 2015 Jun 4. 10.1074/jbc.M115.665521 PubMed 26045559
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