pFG036 + pFG051
(Plasmid #137997)

• Purpose: Transposon mutagenesis plasmid and the cI repressor expression plasmid
• Depositing Lab: Sébastien Rodrigue
• Publication: Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi: 10.1038/s42003-020-01253-0.

Backbone

• Vector backbone: pFG051
• Total vector size (bp): 4434
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin and Tetracycline
• Growth Temperature: 30°C
• Growth Strain(s): DH5apir+
• Growth instructions: Temperature higher than 30°C could lead to denaturation of cI and induce transposition.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Tn5 tnp
• Species: Synthetic
• Insert Size (bp): 1431

Resource Information

• Supplemental Documents:
  • pFG051.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was designed for high density transposon mutagenesis experiment.
It can be delivered by conjugation very efficiently for it's transposon to then be inserted in the recipient DNA.
The donor strain must have pFG036 as well to avoid unwanted transposition.
Indeed, the promoter (pL) in front of the Tn5 transposase encoding gene is repressed by cI expressed from pFG036.
The cloning strain must also have pFG036 to be able to maintain pFG051.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.