pFG036
(Plasmid #137996)

• Purpose: cI repressor expression plasmid
• Depositing Lab: Sébastien Rodrigue
• Publication: Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi: 10.1038/s42003-020-01253-0.

Backbone

• Vector backbone: pFG036
• Total vector size (bp): 4483
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5apir+
• Growth instructions: Growth at temperature over 30 °C could lead to denaturation of cI repressor (ts mutant)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cI
• Alt name: repressor
• Species: Enterobacteria phage lambda
• Insert Size (bp): 714
• Mutation: Thermosensitive
• GenBank ID: J02459.1

Resource Information

• Supplemental Documents:
  • pFG036.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was made to repress the expression of the transposase (pL promoter) in pFG051.
This plasmid has to be in the bacterium to avoid transposition of pFG051's transposon.
It has to be in the donor strain for conjugation to avoid unwanted transposition in the donor.
A strain carrying this plasmid was used to build pFG051.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.