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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13796 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 (+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRRM2
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Alt nameRibonucleotide reductase M2 subunit
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SpeciesH. sapiens (human)
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Insert Size (bp)1170
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GenBank IDBC001886
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Entrez GeneRRM2 (a.k.a. C2orf48, R2, RR2, RR2M)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH rev primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byProtein Tech Group, Inc.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3 RRM2 was a gift from Edward Whang (Addgene plasmid # 13796 ; http://n2t.net/addgene:13796 ; RRID:Addgene_13796) -
For your References section:
RRM2 induces NF-kappaB-dependent MMP-9 activation and enhances cellular invasiveness. Duxbury MS, Whang EE. Biochem Biophys Res Commun. 2007 Mar 2. 354(1):190-196. 10.1016/j.bbrc.2006.12.177 PubMed 17222798