pM2s2AsR_TS-E10
(Plasmid #137944)

• Purpose: E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression
• Depositing Lab: Neel Joshi
• Publication: Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.1021/acssynbio.0c00466.

Backbone

• Vector backbone: pMUT2
• Backbone manufacturer: Native to E. coli Nissle
• Backbone size w/o insert (bp): 3173
• Modifications to backbone: pMUT2 was modified with an insulated cassette with a selection marker
• Vector type: Bacterial Expression, Synthetic Biology ; For use with E. coli Nissle in the mouse gut

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Mach1
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mRFP1
• Species: Synthetic
• Insert Size (bp): 678
• Promoter: J23101

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTTCCTTATCATCTGGCGAATCGG
• 3′ sequencing primer: GAGACGAGACGAGACAGCCTG

Gene/Insert 2

• Gene/Insert name: mWasabi
• Insert Size (bp): 714
• Promoter: pTlpA-C7

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: cattactcgcatccattctcaggc
• 3′ sequencing primer: cctgaattgactctcttccggg

Resource Information

• Supplemental Documents:
  • pM2s2AsR_TS-E10.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry