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Addgene

pPGKFLPobpA Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzyme/enzymes: NcoI, NotI, NcoI&NotI, NcoI&SacII

We expected the following band sizes for this digest: 5kb,5kb, 1.6 & 3.4 kb, and 1.6 & 3.4 kb, respectively. We see the expected pattern.