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PurposeExpression of mouse codon-optimized FLP recombinase with bovine growth hormone polyadenylation (bpA) sequence.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13793 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonena
- Backbone size w/o insert (bp) 3800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLPo
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Alt nameFLP
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Insert Size (bp)1300
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer mPGK-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
FLPo is a mouse codon-optimized FLP.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPGKFLPobpA was a gift from Philippe Soriano (Addgene plasmid # 13793 ; http://n2t.net/addgene:13793 ; RRID:Addgene_13793) -
For your References section:
High-Efficiency FLP and PhiC31 Site-Specific Recombination in Mammalian Cells. Raymond CS, Soriano P. PLoS ONE. 2007 . 2():e162. 10.1371/journal.pone.0000162 PubMed 17225864
Map uploaded by the depositor.
