pM2s2AsG
(Plasmid
#137924)
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PurposeE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 137924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMUT2
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Backbone manufacturerNative to E. coli Nissle
- Backbone size w/o insert (bp) 3173
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Modifications to backbonepMUT2 was modified with an insulated cassette with a selection marker
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Vector typeBacterial Expression, Synthetic Biology ; For use with E. coli Nissle in the mouse gut
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Mach1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSuperfolder GFP
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Alt namesfGFP
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SpeciesSynthetic
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Insert Size (bp)861
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GenBank IDADW83736.1
- Promoter J23101
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GTTCCTTATCATCTGGCGAATCGG
- 3′ sequencing primer GAGACGAGACGAGACAGCCTG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pM2s2AsG was a gift from Neel Joshi (Addgene plasmid # 137924 ; http://n2t.net/addgene:137924 ; RRID:Addgene_137924) -
For your References section:
Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E. coli Nissle 1917. Kan A, Gelfat I, Emani S, Praveschotinunt P, Joshi NS. ACS Synth Biol. 2020 Dec 10. doi: 10.1021/acssynbio.0c00466. 10.1021/acssynbio.0c00466 PubMed 33301298