pTetON-BMP2/7
(Plasmid #137913)

• Purpose: Inducible mammalian co-expression vector for human bone morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, divergent)
• Depositing Lab: Georg Feichtinger
• Publication: Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; discussion 184.

Backbone

• Vector backbone: pTREtight-BI
• Backbone manufacturer: Takara/Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: BMP2
• Species: H. sapiens (human)
• Entrez Gene: BMP2 (a.k.a. BDA2, BMP2A, SSFSC, SSFSC1)
• Promoter: TRE

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: Unknown

Gene/Insert 2

• Gene/Insert name: BMP7
• Species: H. sapiens (human)
• GenBank ID:
• Entrez Gene: BMP7 (a.k.a. OP-1)
• Promoter: TRE

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry