pVAX1-BMP2/7 -
(Plasmid #137912)

• Purpose: Constitutive mammalian co-expression vector for human bone morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, divergent)
• Depositing Lab: Georg Feichtinger
• Publication: Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; discussion 184.

Backbone

• Vector backbone: pVAX1
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: BMP2
• Species: H. sapiens (human)
• Entrez Gene: BMP2 (a.k.a. BDA2, BMP2A, SSFSC, SSFSC1)
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: Unknown

Gene/Insert 2

• Gene/Insert name: BMP7
• Species: H. sapiens (human)
• Entrez Gene: BMP7 (a.k.a. OP-1)
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry