Delta DED FLIP-L D/A
(Plasmid #13791)

• Depositing Lab: Guy Salvesen
• Publication: Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DELTA DED FLIP LONG
• Alt name: FLICE-like inhibitory protein, long form
• Species: H. sapiens (human)
• Insert Size (bp): 890
• Mutation: Delta DED removes first 186 residues Asp to Ala mutation prevents cleavage by caspases
• GenBank ID: DQ893760
• Entrez Gene: CFLAR (a.k.a. CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, cFLIP)
• Tag / Fusion Protein:
  • 8 x His tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry