PylRS-AS
(Plasmid
#137908)
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PurposeFor incorporation of fluorophenylalanine derivatives
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 137908 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEVOL
- Total vector size (bp) 5600
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
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Alt namePylRS/Pyl-tRNA
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SpeciesMethanosarcina mazei
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MutationContaining N346A, C348S mutations
- Promoter pBAD
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBAD-forward
- 3′ sequencing primer pBAD-reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PylRS-AS was a gift from Wenshe Liu (Addgene plasmid # 137908 ; http://n2t.net/addgene:137908 ; RRID:Addgene_137908) -
For your References section:
Genetically encoded fluorophenylalanines enable insights into the recognition of lysine trimethylation by an epigenetic reader. Lee YJ, Schmidt MJ, Tharp JM, Weber A, Koenig AL, Zheng H, Gao J, Waters ML, Summerer D, Liu WR. Chem Commun (Camb). 2016 Oct 18;52(85):12606-12609. doi: 10.1039/c6cc05959g. 10.1039/c6cc05959g PubMed 27711380
