Skip to main content
Addgene

pPS1356
(Plasmid #1379)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1379 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRS315
  • Backbone size w/o insert (bp) 6018
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NPL3
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3500
  • Entrez Gene
    NPL3 (a.k.a. YDR432W, MTR13, MTS1, NAB1, NOP3)
  • Tag / Fusion Protein
    • Myc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

3.5kb ScaI fragment ligated into SmaI digested vector (CEN plasmid). See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS1356 was a gift from Pamela Silver (Addgene plasmid # 1379 ; http://n2t.net/addgene:1379 ; RRID:Addgene_1379)

  • For your References section:

    Arginine methylation facilitates the nuclear export of hnRNP proteins. Shen EC, Henry MF, Weiss VH, Valentini SR, Silver PA, Lee MS. Genes Dev 1998 Mar 1;12(5):679-91. 10.1101/gad.12.5.679 PubMed 9499403
Related items: