pMDC32B-AtTAS1c-D2-B/c
(Plasmid #137884)

• Purpose: Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+]
• Depositing Lab: Alberto Carbonell
• Publication: Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 5836195. doi: 10.1093/nar/gkaa343.

Backbone

• Vector backbone: pMDC32
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size w/o insert (bp): 10141
• Total vector size (bp): 12592
• Vector type: Plant Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: AtTAS1c-D2-B/c
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2451
• Mutation: A. thaliana TAS1c precursor sequence including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites downstream the 3'D1[+] position. The BsaI site in the ccdB gene was mutated to block the site.
• Promoter: 2x35S

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: RC-attB1: ACAAGTTTGTACAAAAAAGCAGGCT
• 3′ sequencing primer: RC-attB2: ACCACTTTGTACAAGAAAGCTGGGT

Resource Information

• Supplemental Documents:
  • pMDC32B-AtTAS1c-D2-Bc.gb
  • Cloning protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry