pCAG-Flpe:GFP
(Plasmid #13788)

• Purpose: Mammalian expression of enhanced form of Flp recombinase fused to GFP
• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 4779
• Vector type: Mammalian Expression ; Flp/FRT

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Flpe-GFP fusion protein
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2024
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The coding sequence of Flpe was amplified by PCR using pCMVscript-FlpeERT2 (Hunter et al. Genesis 41, 99-109 (2005)) obtained from Dr. S. Dymecki (Harvard Medical School) as a template. GFP was from pEGFP-N1 (Clontech).
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Kozak consensus sequence was added before the start ATG. Internal EcoRI site was destroyed by introducing a silent mutation.