pCALSL-mir30
(Plasmid #13786)

• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 5555
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mir30 cassette
• Species: H. sapiens (human)
• Insert Size (bp): 205

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The mir30 cassette was derived from pSM2 (Open Biosystems).
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cre recombinase-dependent inducible RNAi vector. shRNA is expressed only in the presence of Cre.
Haipin DNA can be cloned into the XhoI/EcoRI site. A protocol for hairpin DNA design and cloning was reported by Gregory Hannon lab http://hannonlab.cshl.edu/publications/shrt_hrpin_RNA.pdf (Paddison et al. Nat. Methods 1, 163-167 (2004)).

Addgene's quality control sequencing finds a few nucleotides of discrepancy with the depositor's provided full plasmid sequence. These changes are not thought to affect plasmid function.