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PB CAG-sfGFP-iCre-ERT2
(Plasmid #137856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom
  • Backbone manufacturer
    Hyung-song Nam, Capecchi laboratory
  • Vector type
    Mammalian Expression, Cre/Lox ; piggyBac

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP-iCre-ERT2
  • Species
    Synthetic

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    piggyBac ITR's were synthesized from sequences in the NCBI database. CAG promoter is from pCAGGS obtained from BCCM/LMBP. sfGFP is from Sandia Biotech. iCre is from Rolf Sprengel. ERT2 is from Pierre Chambon. WPRE is from Miguel Ramalho-Santos. bGH pA is from Neal Copeland.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip.

The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35) In short, it is not visible to the eyes, but can be detected with flow cytometry.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB CAG-sfGFP-iCre-ERT2 was a gift from Mario Capecchi (Addgene plasmid # 137856 ; http://n2t.net/addgene:137856 ; RRID:Addgene_137856)
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