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pCMV-HA_N-human Prkd2
(Plasmid #137850)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137850 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-HA-N
  • Backbone size w/o insert (bp) 3782
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Protein kinase d2
  • Alt name
    Prkd2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2637
  • GenBank ID
    25865
  • Entrez Gene
    PRKD2 (a.k.a. HSPC187, PKD2, nPKC-D2)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)
  • 5′ sequencing primer ACCGAGATCTCTCGAATGGCCACCGCCCCCTCTTATCC
  • 3′ sequencing primer TGGATCCCCGCGGCCTCAGAGAACACTGATGCGCTCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-HA_N-human Prkd2 was a gift from Bruce Beutler (Addgene plasmid # 137850 ; http://n2t.net/addgene:137850 ; RRID:Addgene_137850)

  • For your References section:

    Mutual inhibition between Prkd2 and Bcl6 controls T follicular helper cell differentiation. Misawa T, SoRelle JA, Choi JH, Yue T, Wang KW, McAlpine W, Wang J, Liu A, Tabeta K, Turer EE, Evers B, Nair-Gill E, Poddar S, Su L, Ou F, Yu L, Russell J, Ludwig S, Zhan X, Hildebrand S, Li X, Tang M, Murray AR, Moresco EMY, Beutler B. Sci Immunol. 2020 Jan 24;5(43):eaaz0085. doi: 10.1126/sciimmunol.aaz0085. 10.1126/sciimmunol.aaz0085 PubMed 31980486
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