pUF-LbCpf1-pre-gRNA
(Plasmid #137848)

• Purpose: LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.
• Depositing Lab: Josh Beck
• Publication: Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi: 10.1111/cmi.13168.

Backbone

• Vector backbone: pUF-Cas9-pre-sgRNA
• Vector type: CRISPR
• Selectable markers: yDHODH

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LbCpf1-NLS-FLAG and gRNA cassette
• Alt name: Cpf1
• Species: Lachnospiraceae bacterium ND2006 and P. falciparum
• Insert Size (bp): 6308

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pUF-LbCpf1-pre-gRNA
• A portion of this plasmid was derived from a plasmid made by: Zetsche et al, Addgene #69988

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry