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PA-Bongwoori-R3
(Plasmid #137838)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137838 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Thermo Fischer (Invitrogen)
  • Backbone size w/o insert (bp) 5526
  • Total vector size (bp) 6969
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Photoactivatable_Bongwoori-R3
  • Alt name
    PA-Bongwoori-R3
  • Species
    Ciona Intestinalis (for voltage-sensing domain), Aequorea victoria (for GFP variant)
  • Insert Size (bp)
    1443
  • Mutation
    L64F, T65S, V163A, T203H, A227D
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer CMV_forward
  • 3′ sequencing primer BGH_reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PA-Bongwoori-R3 was a gift from Bradley Baker (Addgene plasmid # 137838 ; http://n2t.net/addgene:137838 ; RRID:Addgene_137838)

  • For your References section:

    Engineering Photoactivatability in Genetically Encoded Voltage and pH Indicators. Lee S, Song YK, Baker BJ. Front Cell Neurosci. 2019 Oct 29;13:482. doi: 10.3389/fncel.2019.00482. eCollection 2019. 10.3389/fncel.2019.00482 PubMed 31736711
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