PA-ecliptic pHluorin
(Plasmid #137837)

• Purpose: A photoactivatable GEVI variant originated from Bongwoori-R3
• Depositing Lab: Bradley Baker
• Publication: Lee et al Front Cell Neurosci. 2019 Oct 29;13:482. doi: 10.3389/fncel.2019.00482. eCollection 2019.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Thermo Fischer (Invitrogen)
• Backbone size w/o insert (bp): 5526
• Total vector size (bp): 6243
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Photoactivatable-ecliptic pHluorin
• Alt name: PA-ecliptic pHluorin
• Species: Aequorea victoria
• Insert Size (bp): 717
• Mutation: L64F, T65S, V163A, T203H
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: CMV_forward
• 3′ sequencing primer: BGH_reverse

Resource Information

• Supplemental Documents:
  • pcDNA3.1-PA-ecliptic pHluorin T203H_confirmed_.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry