3NES-CS(G)-tTA-CS(G)-3NES
(Plasmid
#137836)
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PurposeSoluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequences
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 137836 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1/HygroR(+)
- Backbone size w/o insert (bp) 4318
- Total vector size (bp) 1260
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Modifications to backboneModified from pcDNA3.1/HygroR(+) to lack HygroR.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3NES-CD(G)-tTA-CS(G)-3NES
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SpeciesH. sapiens (human)
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Insert Size (bp)1260
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
- 3′ sequencing primer TAGAAGGCACAGTCGAGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/863530v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3NES-CS(G)-tTA-CS(G)-3NES was a gift from Joshua Leonard (Addgene plasmid # 137836 ; http://n2t.net/addgene:137836 ; RRID:Addgene_137836) -
For your References section:
Computation-guided optimization of split protein systems. Dolberg TB, Meger AT, Boucher JD, Corcoran WK, Schauer EE, Prybutok AN, Raman S, Leonard JN. Nat Chem Biol. 2021 May;17(5):531-539. doi: 10.1038/s41589-020-00729-8. Epub 2021 Feb 1. 10.1038/s41589-020-00729-8 PubMed 33526893