Gq sensor
(Plasmid
#137810)
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PurposeA FRET sensor for Gq activation, encoded on a single plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 137810 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGq sensor
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SpeciesH. sapiens (human)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Gq sensor was a gift from Dorus Gadella (Addgene plasmid # 137810 ; http://n2t.net/addgene:137810 ; RRID:Addgene_137810) -
For your References section:
Quantitative co-expression of proteins at the single cell level--application to a multimeric FRET sensor. Goedhart J, van Weeren L, Adjobo-Hermans MJ, Elzenaar I, Hink MA, Gadella TW Jr. PLoS One. 2011;6(11):e27321. doi: 10.1371/journal.pone.0027321. Epub 2011 Nov 17. 10.1371/journal.pone.0027321 PubMed 22114669