PiggyBac-G13-sensor
(Plasmid
#137809)
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PurposeA FRET sensor for G13 activation, encoded on a single plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 137809 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePiggyBac
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGalpha13 sensor
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SpeciesH. sapiens (human)
- Promoter CMV
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PiggyBac-G13-sensor was a gift from Dorus Gadella (Addgene plasmid # 137809 ; http://n2t.net/addgene:137809 ; RRID:Addgene_137809) -
For your References section:
Imaging of Genetically Encoded FRET-Based Biosensors to Detect GPCR Activity. Bordes L, Chavez-Abiega S, Goedhart J. Methods Mol Biol. 2021;2268:159-178. doi: 10.1007/978-1-0716-1221-7_11. 10.1007/978-1-0716-1221-7_11 PubMed 34085268