pNrl-Cre
(Plasmid #13780)

• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104

Backbone

• Vector backbone: na
• Backbone size w/o insert (bp): 3100
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nrl promoter-Cre
• Alt name: Nrl
• Species: M. musculus (mouse)
• Insert Size (bp): 4308
• Entrez Gene: Nrl (a.k.a. D14H14S46E)
• Tag / Fusion Protein:
  • Cre (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The coding sequence of Cre was amplified by PCR using pxCANCre (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo) as a template.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mouse Nrl promoter was isolated by genomic PCR using B6 mouse genomic DNA as a template.
The Nrl promoter-Cre is specific for rod photorecetor cells in the retina.
Cre has a Myc-tag at its C-terminus.