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RnAT1aR DRY->AAY-mCherry
(Plasmid #137783)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137783 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rat At1a receptor; DRY mutant
  • Species
    R. norvegicus (rat)
  • Mutation
    DRY -> AAY
  • Entrez Gene
    Agtr1a (a.k.a. AT1, AT1A, AT1R, Agtr1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RnAT1aR DRY->AAY-mCherry was a gift from Dorus Gadella (Addgene plasmid # 137783 ; http://n2t.net/addgene:137783 ; RRID:Addgene_137783)

  • For your References section:

    Not So Dry After All: DRY Mutants of the AT1A Receptor and H1 Receptor Can Induce G-Protein-Dependent Signaling. Pietraszewska-Bogiel A, Joosen L, Chertkova AO, Goedhart J. ACS Omega. 2020 Feb 3;5(6):2648-2659. doi: 10.1021/acsomega.9b03146. eCollection 2020 Feb 18. 10.1021/acsomega.9b03146 PubMed 32095688
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